The paper presents calibration method of the spectrometer HR-2000, with the usage of heusler tubes with three inert gases. Equipment for the spectrometer calibration is described. Work peculiarities with heusler tubes and fiberoptic spectrometer are described. Three emission spectra of helium, neon and krypton inert gases are obtained. Results of the calibration are presented. Results of the wavelength drift comparison of the scale before and after calibration are presented. Conclusions about instrument errors of the recording spectra are made. It has been shown that self-calibration is required to improve the accuracy of the spectral analysis.